Recently, Dr. Jennifer Redig wrote an intriguing article titled, “Where the hell did my signal go?” AKA The Problems (and Uses) of ‘Photobleaching’ in Microscopy and Imaging.
As the title suggests, photobleaching can be quite useful. The article highlights this with an example of fluorescent imaging with tissue, where photobleaching helps reduce autofluorescence and thus background noise.
It’s fantastic to see articles like these raising awareness about the uses of photobleaching. Peira developed the HistoSAFE 700, an instrument for controlled irradiation exposure. This device is primarily used in histology and has been scientifically tested by the Antwerp Centre for Advanced Microscopy (ACAM) at the University of Antwerp.