Autofluorescence in histology is the natural light emitted by biological
structures such as mitochondria and lysosomes when they absorb light. This phenomenon can hinder the detection of specific fluorescent signals, particularly when these signals are faint. While optimization methods such as autofluorescence quenching agents can help minimize autofluorescence, they are often cumbersome and yield inconsistent results.
However Peira proposes with the HistoSAFE 700 a simple method for reducing autofluorescence in fluorescence microscopy based on a integrated photo-irradiation approach.
Simple and effective photo-irradiation
The Peira HistoSAFE 700 consolidates the photobleaching approach into an integrated system, addressing the issue of background non-specific fluorescence from fluorophores due to prolonged light exposure. Utilizing advanced radiation technology and experiment-backed knowledge, the Peira HistoSAFE 700 offers simple and safe background removal. Just insert slides and cell culture dishes via a holder tray and execute the radiation cycle. Easy!
Before Photo-Irradiation  Human colon Courtesy of ACAM | Peira HistoSAFE-700 stand-alone module | After Photo-Irradiation Human colon Courtesy of ACAM |
| Autofluorescence a complicating factor for quantitative immunofluorescence assays and FISH and is inherent to many tissues. | HistoSAFE-700 a photo-irradiation device capable of removing autofluorescence from samples of different origin encompassing the entire spectrum. | Application highly reproducible, tunable, inexpensive, non-destructive, it does not compromise the antigenicity and requires minimal interaction. |
Why Peira HistoSAFE 700?
This method is effortless, tunable, cost-effective, non-destructive, and preserves sample antigenicity with minimal user interaction. Furthermore, the Peira HistoSafe-700 offers several advantages for histological processing and staining:
- The Peira HistoSAFE 700 is a stand-alone system designed to seamlessly integrate into existing laboratory workflows to remove endogenous fluorescent signals.
- Easy to use and the HistoSAFE 700 simplifies the histological processing, requiring fewer steps compared to traditional methods, which accelerates the overall process.
- This system uses no solvents, thus reducing environmental impact, making it safer for lab personnel and reducing the ecological footprint.
- Enhanced staining results: It improves the staining quality of tissues, making it easier to visualize fluorescent signals.
- Potential use in iterative re-staining protocols.
- Peira HistoSAFE 700 is compatible with all tissue processors, automated stainers, and mounting media
These features make the Peira HistoSAFE 700 a valuable tool for laboratories focused on histological analysis.
“Just plug in the system and you
Rony Nuydens, Senior Scientist, Pharmaceutical development
are ready to go. If you are done, there are no solvents
or any other waste to deal with.”
Start “HistoSaving”
Download the product information brochure or contact us for more information.